DDP-sensitivity-related genes in 10 lung cancer cell lines.
- Author:
Chun-hong LI
1
;
Li CAI
;
Xue-song CHEN
;
Qing-wei MENG
;
Guang-jie SUI
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents; pharmacology; Carcinoma, Non-Small-Cell Lung; genetics; metabolism; pathology; Cell Line, Tumor; Cisplatin; pharmacology; Drug Resistance, Neoplasm; Gene Expression Profiling; methods; Gene Expression Regulation, Neoplastic; Humans; Lung Neoplasms; genetics; metabolism; pathology; Oligonucleotide Array Sequence Analysis; methods; Small Cell Lung Carcinoma; genetics; metabolism; pathology
- From: Chinese Journal of Oncology 2008;30(6):418-421
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo search for genes related to cisplatin (DDP) sensitivity in small-cell lung cancer (SCLC) and non-small-cell lung cancer (NSCLC) cell lines.
METHODSThe sensitivity of 4 SCLC lines and 6 NSCLC lines to DDP was evaluated by MTT assay. The expression of 1291 genes related to DDP-sensitivity in the 10 cell lines was measured by cDNA macroarray and the relationship between genes and DDP-sensitivity was analyzed.
RESULTS20 genes were negatively related to DDP-sensitivity in the SCLC and NSCLC cell lines, including Metallothionein, Cathepsin B, TIMP1, TNF-R1, TGF beta-induced 68 000, Cathepsin L, Galectin-1, Annexin 11, PAI-1, IGFBP4, UPAR, Jagged, CD13, alpha 1 A-AR, EphA2 (Eck), APC, RhoC, Fibromodulin, GATA-6 and HSC 70, while only procoagula and MDM2 were positively related to DDP-sensitivity in the SCLC and NSCLC cell lines. 10 genes were negatively related to DDP-sensitivity in the SCLC cell lines, including VHL, MMP-7, Elongin A, GSK-3 beta, SLC, Galectin-3, integrin beta 5, moesin, IKK beta, and ETV 1, while only AT2 was positively related to DDP-sensitivity in the SCLC cell lines. 10 genes were negatively related to DDP-sensitivity in the NSCLC cell lines, including Clusterin, FG FR-2, Thrombospondin 1, HSP 32, Lactate dehydrogenase A, P300, Thymosin beta l0, CD81, C/EBP gamma, Rak, while only CaMKK and TPA were positively related to DDP-sensitivity in the NSCLC cell lines.
CONCLUSIONThere were 45 genes related to DDP-sensitivity in 10 lung cancer cell lines. There were 22 co-expressed genes in both SCLC and NSCLC cell lines, and only 11 and 12 genes expressed in the SCLC and NSCLC cell lines, respectively.