Expression of SV40Tag, Rb and IRS-1 in glioma detected by tissue microarray and their relation with tumorigenesis and progression of gliomas.
- Author:
Zhi-yuan QIAN
1
;
Yin-yan WU
;
Qiang HUANG
;
De-zhong ZHAI
;
Qing ZHU
;
Ai-dong WANG
;
Hong-mei HUO
;
Qing LAN
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; Animals; Antigens, Polyomavirus Transforming; metabolism; Brain; metabolism; pathology; Brain Neoplasms; metabolism; pathology; Cell Line, Tumor; Child; Child, Preschool; Female; Gene Expression Regulation, Neoplastic; Glioma; metabolism; pathology; Humans; Insulin Receptor Substrate Proteins; metabolism; Male; Meningioma; metabolism; pathology; Mice; Middle Aged; Neoplasm Transplantation; Rats; Rats, Sprague-Dawley; Retinoblastoma Protein; metabolism; Tissue Array Analysis; Young Adult
- From: Chinese Journal of Oncology 2008;30(6):432-436
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the expression of SV40Tag, Rb and IRS-1 in gliomas and to identify their function in gliomagenesis and progression.
METHODSTissue microarrays were constructed containing 118 samples including human glioma and meningioma, experimental glioma, and normal human brain tissue. The expression of SV40Tag, Rb, IRS-1, SV40Tag combined with Rb, and SV40Tag combined with IRS-1 were assayed by immunofluorescence or immunohistochemical techniques. The expression ratio and level were analyzed.
RESULTSThe expressions of SV40Tag, Rb and IRS-1 were detected in gliomas and benign brain tumors. Their positive expression rate in glioma was 65.9%, 64.6% and 48.8%, respectively, with a statistically non-significant difference between the malignant and benign brain tumors. The malignant degree was positively correlated with SV40Tag and IRS-1, but negatively correlated with Rb expression. The combined expression rate of SV40Tag and Rb was 51.2%, and the combined expression rate of SV40Tag and IRS-1 was 40.2%. In the normal human brain tissue only the expression of Rb (77.8%, 7/9) and IRS-1 (22.2%, 2/9) were detected, but expression of SV40Tag could not be observed.
CONCLUSIONOur findings that no expression of SV40Tag was observed in normal human brain tissue indicates that expression of SV40Tag may play an important role in the pathogenesis of glioma. It may be assumed that after SV40 virus invading human body, Rb disfunction and IRS-1 activation promote the malignant transformation of cells, which could be one of important factors in pathogenesis and procession of glioms.
