Apoptosis induced by NNAMB, a novel polyamine conjugate, in human erythroleukemia K562 cells and its mechanism.
- Author:
Song-Qiang XIE
1
;
Ying-Liang WU
;
Guang-Chao LIU
;
Peng-Fei CHENG
;
Min-Wei WANG
;
Yuan-Fang MA
;
Jin ZHAO
;
Chao-Jie WANG
Author Information
- Publication Type:Journal Article
- MeSH: Anthracenes; pharmacology; Apoptosis; drug effects; Caspase 3; metabolism; Caspase 8; metabolism; Caspase 9; metabolism; Cell Cycle; drug effects; Cell Proliferation; drug effects; Cytochromes c; metabolism; Humans; K562 Cells; Membrane Potential, Mitochondrial; drug effects; Polyamines; pharmacology; Spermidine; analogs & derivatives; pharmacology
- From: Chinese Journal of Oncology 2008;30(7):490-493
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the apoptosis-inducing effects of NNAMB, a novel polyamine conjugate, in erythroleukemia K562 cells and its molecular mechanism.
METHODSCell viability was assessed by MTT assay and trypan blue dye exclusion method. The cell morphology was observed by fluorescence microscopy. The cell cycle distribution, apoptosis and mitochondrial membrane potential were measured by flow cytometry. The expression of caspase-3, -8, -9, cytochrome c in the K562 cells was detected by Western blot.
RESULTSNNAMB inhibited the proliferation of K562 cells. The cells treated with NNAMB showed a typical apoptotic morphology, Sub-G1 peak and loss of mitochondrial membrane potential. Western blot assay showed that NNAMB increased the expression of caspase-3, -9, cytochrome c but not caspase-8 in a dose-and time-dependent manner.
CONCLUSIONNNAMB induces apoptosis via mitochondrial pathway in K562 cells.
