Effect of microRNA143 expression on cell proliferation in colonic carcinoma.
- Author:
Hong LIU
1
;
Su-Zhan ZHANG
;
Shan-Rong CAI
;
Jia-Ping PENG
;
Shu ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma; genetics; metabolism; pathology; Cell Line, Tumor; Cell Proliferation; Colonic Neoplasms; genetics; metabolism; pathology; Down-Regulation; Genes, ras; Genetic Vectors; Humans; MicroRNAs; genetics; metabolism; Plasmids; RNA, Messenger; metabolism; Transfection; ras Proteins; metabolism
- From: Chinese Journal of Oncology 2008;30(7):498-501
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of microRNA143 on cell proliferation and K-ras expression in colorectal carcinoma.
METHODSNorthern blot was used to examine the expression of miR-143 in colorectal carcinoma and adjacent normal tissues. A miR-143 expression vector was constructed and transfected into a human colon adenocarcinoma cell line SW480. Cell proliferation was evaluated by MTT assay. RT-PCR and Western blot were used to examine the expression of K-ras oncogene in transfected cells.
RESULTSThe level of mature miR-143 was lower in tumors compared with adjacent normal tissues in 81% of colorectal carcinoma specimens. In transfected cells, the increased accumulation of miR-143 inhibited the cell proliferation, and resulted in approximately 40.3% decrease of K-ras protein levels, but had no effect on level of K-ras mRNA.
CONCLUSIONThe increased accumulation of miR-143 inhibits the proliferation of transfected cells, and results in down-regulation of K-ras protein in colorectal carcinoma.
