Relationship between combined multigene detection and response to adjuvant chemotherapy in early-stage non-small cell lung cancer.
- Author:
Hong PAN
1
;
Li LI
;
Chuan-Tian ZUO
;
Nai-Quan MAO
;
Fa-Long CHEN
;
Wei ZHANG
;
Bu-Jian TANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Carcinoma, Non-Small-Cell Lung; drug therapy; metabolism; pathology; surgery; Chemotherapy, Adjuvant; DNA-Binding Proteins; metabolism; Endonucleases; metabolism; Female; Follow-Up Studies; Humans; Inhibitor of Apoptosis Proteins; Logistic Models; Lung Neoplasms; drug therapy; metabolism; pathology; surgery; Male; Microtubule-Associated Proteins; metabolism; Middle Aged; Neoplasm Staging; Survival Rate; Tissue Array Analysis; Vascular Endothelial Growth Factor A; metabolism; bcl-2-Associated X Protein; metabolism
- From: Chinese Journal of Oncology 2008;30(7):528-531
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the relationship between combined multigene detection and response to adjuvant chemotherapy and prognosis in early-stage non-small cell lung cancer (NSCLC).
METHODSTissue microarray was prepared from samples of 86 cases of early-stage NSCLC who received adjuvant chemotherapy after radical surgery. The expressions of caspase-3, Fas, bax, bcl-2, survivin, PCNA, Ki67, MGMT, p53, p63, p73, p16, p27, VEGF, nm23, P-gp, MRP, LRP, GST-pi, Topo II, c-myc, cyclin-D1, Her-2, Cox-2, Ku70, Ku80, DNA-PKcs, ERCC1, MSH2, BCRP proteins were detected using immunohistochemical two-step method.
RESULTSThe positive rate of the 30 genes in lung cancer tissue were 27.9% - 91.9%, respectively. By univariate analysis, the expression of 8 genes was shown to be related with SCLC adjuvant chemotherapy. The cases with higher expression of survivin, P-gp, LRP, Ki67, p53, ERCC1 and lower expression of bax,VEGF had worse prognosis. By logistic regression analysis, the ERCC1, survivin, bax and VEGF were a marker group. Multivariate analysis showed the predict value of the response to adjuvant chemotherapy in early-stage NSCLC was 96.5%.
CONCLUSIONSurvivin, ERCC1, bax and VEGF are an ideal marker group to predict the effect of adjuvant chemotherapy in early-stage NSCLC.
