Salinomycin Enhances the Apoptosis of T-cell Acute Lymphoblastic Leukemia Cell Line Jurkat Cells Induced by Vincristine.
- Author:
Ping-Ping LIU
1
;
Jin-Can ZHU
2
;
Ge-Xiu LIU
2
;
Chao-Xiang SHUI
1
;
Xiao-Mei LI
1
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Caspase 3; Caspase 8; Cell Proliferation; Flow Cytometry; Humans; Jurkat Cells; Precursor T-Cell Lymphoblastic Leukemia-Lymphoma; Pyrans; Vincristine
- From: Journal of Experimental Hematology 2015;23(3):653-657
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThis study was aimed to investigate the effect of salinomycin combined with vincristine on the proliferation and apoptosis of Jurkat cells and its possible mechanisms.
METHODSThe proliferation of Jurkat cells was examined by CKK-8 assay. Flow cytometry was used to assess cellular apoptosis. Levels of BCL-2, caspase-3, and caspase- 8 were measured by Western blot.
RESULTSThe salinomycin or vincristine, either alone or in combination, inhibited the proliferation of Jurkat cells in a dose-dependent manner. Salinomycin combined with vincristine produced more obveous inhibition of cell proliferation than either compound used alone (P<0.05). Western blot analysis showed that the combined use of Sal and VCR reduced the expression of BCL-2 protein, and increased expression of caspase 3 and caspase 8 protein, more significantly. Furthermore, combination of Sal and VCR synergistally promoted apoptosis of the Jurkat cells (P<0.05).
CONCLUSIONThe combination of salinomycin and vincristine synergistically inhibits proliferation and promotes apoptosis of T-cell acute lymphoblastic leukemia Jurkat cells.
