Correlation of WT1 and VEGF Expression with Angiogenesis in Bone Marrow Biopsies of Multiple Myeloma Patients.

Guo-Jie LI; Hui DING; Liu YANG

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Correlation of WT1 and VEGF Expression with Angiogenesis in Bone Marrow Biopsies of Multiple Myeloma Patients.

Author: Guo-Jie LI ¹ ; Hui DING ² ; Liu YANG ²
Author Information

1. Department of Hematology, Huaihe Hospital of Henan University, Kaifeng 475001, Henan Province, China. E-mail: 455298676@qq.com.
2. Department of Hematology, Huaihe Hospital of Henan University, Kaifeng 475001, Henan Province, China.
Publication Type:Journal Article
MeSH: Biopsy; Bone Marrow; Humans; Multiple Myeloma; Neovascularization, Pathologic; Vascular Endothelial Growth Factor A; WT1 Proteins
From: Journal of Experimental Hematology 2015;23(3):733-736
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo investigate the relationship of WT1 and VEGF expression with angiogenesis in bone marrow biopsies of multiple myeloma patients.
METHODSVEGF, WT1 expression and microvessel density (MVD) of 62 cases of multiple myeloma and 10 normal bone marrow tissue were detected by in situ hybridization and immunohistochemistry SP method.
RESULTSMicrovessel density (MVD) of the control group was (45±6)/visual field, and while MVD of the multiple myeloma group was (84±26)/sight, and statistical analysis showed that MVD in multiple myeloma group was significantly higher than that in control group (P<0.05); in 62 cases of multiple myeloma the VEGF positive rate was 51.6% (32/62), and MVD in VEGF-positive group was significantly higher than that in the negative group (P<0.05). WT1 positive rate was 30.6% (19/62), and MVD in WT1-positive group was significantly higher than that in the negative group (P<0.05). And statistical analysis showed that WT1 expression significantly correlated with VEGF expression (P<0.05).
CONCLUSIONWT1 high expression of multiple myeloma bone tissue can up-regulate VEGF expression and promote angiogenesis.