Supporting Effect of Umbilical Cord Blood-Derived Mesenchymal Stem Cells on CD34+ Cell Proliferation and Its Mechanism.
- Author:
Zhi-Juan XU
1
;
Li-Xia SHENG
1
;
Yan-Ru LOU
1
;
Qi-Tian MU
1
;
Yi ZHANG
1
;
Yi-Sheng ZHANG
1
;
Gui-Fang OUYANG
2
Author Information
- Publication Type:Journal Article
- MeSH: Antigens, CD34; Cell Proliferation; Coculture Techniques; Cytokines; Fetal Blood; Humans; Mesenchymal Stromal Cells
- From: Journal of Experimental Hematology 2015;23(3):802-808
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the ability of UCB-derived MSCs to support the expansion of HSCs ex vivo and the possible mechanisms involved in this process.
METHODSHSCs from UCB were co-cultured with UCB-derived MSCs for 14 days, and then the total number of HSCs and colony-forming units (CFU) were detected. Cytokines levels of MSCs supernatant were analyzed using ELISA.
RESULTSThe proliferation rate of HSCs co-cultured with MSCs was significantly higher than that of cultured HSCs alone (P<0.05). Furthermore, the addition of exogenous cytokines to the culture system significantly increased the proliferation rate of HSCs (P<0.05). MSCs had secretion of many cytokines, including GM-CSF, IL-7, IL-8, IL-11, SCF and SDF-1α.
CONCLUSIONUCB-derived MSCs as a feeder layer can be an alternative approach for ex vivo expansion of HSCs, and the cytokines by secreted UCB-MSCs may mediate the supportive role of MSC to HSC proliferation.
