Analysis of the Physiological Activities and Functions in Vitro of Apheresis Platelets during Storage.

Huan-ming XU; Dong-yang Zhao; Yi-wen Hao; Ning-ning Gao; Ji-xiang Cui

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Analysis of the Physiological Activities and Functions in Vitro of Apheresis Platelets during Storage.

Author: Huan-Ming XU ¹ ; Dong-Yang ZHAO ² ; Yi-Wen HAO ³ ; Ning-Ning GAO ⁴ ; Ji-Xiang CUI ⁵
Author Information

1. Department of Blood Transfusion, The Fourth Hospital Affiliated to China Medical University, Shenyang 110032, Liaoning Province, China.
2. Liaoning Provincial Blood Center, Shengyang 110044, Liaoning Province, China.
3. Department of Blood Transfusion, The First Hospital Affiliated to China Medical Universoty, Shenyang 110001, Liaoning Province, China.
4. Department of Utrasonics, The First Hospital Affiliated to China Medical Universoty, Shenyang 110001, Liaoning Province, China.
5. Department of Blood Transfusion, The Fourth Hospital Affiliated to China Medical University, Shenyang 110032, Liaoning Province, China. E-mail: 317780708@qq.com.
Publication Type:Journal Article
MeSH: Blood Platelets; Blood Preservation; Humans; In Vitro Techniques; Platelet Count; Plateletpheresis; Thrombelastography
From: Journal of Experimental Hematology 2015;23(3):856-860
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo investigate the changes of physiological activities and functions in vitro of apheresis platelets during storage.
METHOD17 units of apheresis platelets were randomly chosen and stored at 20 °C to 24 °C with agitation. Platelet counting (Plt), mean platelet volume (MPV), blood gases, pH value, glucose (Glu) concentration, lactate (LA) concentration, LDH concentration, thromboelastogram (TEG), hypotonic shock response (HSR), CD62p expression rate and anew expression rate were measured on days 0, 1, 3, 5 after platelet storage. Changes of physiological activities and functions in vitro were systematically evaluated by above-mentioned indexes.
RESULTSDuring storage, Plt, MPV and HSR were not significantly changed; but pH value, blood gases, Glu, LA, LDH, HSR, expression rate of CD62p and anew expression rate were significant differenty. Among thromboelastogram indexes, R value increased obviously with prolongation of storage time; K value and αAngle were not significantly changed; MA was not significantly changed on day 1 and 3, but was slightly increase on day 5.
CONCLUSIONThe physiological activities and functions in vitro of apheresis platelets are kept well during storage. For clinical transfusion of apheresis platelet during storage, clinical effect of transfusione is not influenced.