Effect of PKF118-310 on Cell Cycle and Proliferation of K562 Cells and Its Mechanism.
- Author:
Dan-Yu WANG
1
;
Wei-Ming LI
2
;
Meng-Di JING
1
;
Hai-Yan CUI
1
;
Bo LU
1
;
Xin WANG
1
;
Ze-Lin LIU
3
Author Information
- Publication Type:Journal Article
- MeSH: Caspase 3; Cell Cycle; Cell Proliferation; Humans; K562 Cells; Pyrimidinones; Triazines; beta Catenin
- From: Journal of Experimental Hematology 2015;23(4):989-993
- CountryChina
- Language:Chinese
-
Abstract:
UNLABELLEDObjetive: To investigate the effects of PKF118-310 on cell cycle and proliferation of K562 cell lines and its mechanism.
METHODSAfter treatment of PKF118-310 with different concentration, the proliferation inhibition on K562 cell lines was detected by MTT, the existance of β-catenin and TCF-4 in the cells was observed by immunohistochemistry. The change of the cell cycle was detected by flow cytometry. The expressions of caspase-3, β-catenin, TCF and BCL-9 were detected by Western blot.
RESULTSPKF118-310 can inhibit the proliferation of K562 cell line by S phase blocking. The β-catenin and TCF in the cells were observed by immunohistochemistry. After treating this cell line with PKF118-310 of different concentrations for 72 h, the expression level of caspase-3 increased, the expression levels of β-catenin, TCF and BCL-9 significantly decreased.
CONCLUSIONPKF118-310 induces cycle arest of K562 cells at the S phase and inhibits the proliferation of these cells through decreasing β-catenin/TCF/BCL-9 thrascriptional activity.
