Inhibitory Effect of Curcumin on Proliferation of CD34(+) Acute Myeloid Leukemia Cells and Its Mechanism.
- Author:
Jia RAO
1
,
2
;
. E-mail: RAOJIA2004126@126.COM.
;
Rong-Yan ZHANG
1
;
Guo-An CHEN
1
;
Fei LI
1
;
Ren-Wei HUANG
3
Author Information
- Publication Type:Journal Article
- MeSH: Antigens, CD34; Apoptosis; Cell Cycle; Cell Line, Tumor; Cell Proliferation; Cell Survival; Curcumin; Down-Regulation; Humans; Leukemia, Myeloid, Acute; NF-kappa B; Transcription Factor RelA
- From: Journal of Experimental Hematology 2015;23(4):1005-1008
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the inhibitory effect of curcumin on proliferation of CD34(+) acute myeloid leukemia cells and its mechamism.
METHODSKG1a and Kasumi-1cell lines were treated with curcumin of different concentrations (0, 40, 60, 80 µmol/L). The effect of curcumin on cell viability and proliferation was detected by trypan blue staining and cell count. The effect of curcumin on distribution of NF-κB P65 subunit was analyzed by immunofluorescence and Western blot.
RESULTSThe curcumin inhibited proliferation of KG1a and Kasumi-1 cells in a dose-dependent manner. Western blotting showed that curcumin led to significant down-regulation of NF-κB P65 nuclear protein expression. Immunofluorescence assay showed that treatment with 40 µmol/L of curcumin for 48h suppressed the nuclear translocation of NF-κB p65 in KG1a and Kasumi-1 cells.
CONCLUSIONThe curcumin suppresses cell growth of KG1a and Kasumi-1 cells, its mechanism may be related to inhibitory effect of curcumin on NF-κB p65 nucleus protein.
