Effect of human colon carcinoma-associated fibroblasts on biological behavior of colon carcinoma Lovo cells.
- Author:
Xin-lin WU
1
;
Kai-jin LIN
;
Lin SHI
;
Xiu-lan SU
;
Ming-xing HOU
;
Pei-de DONG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Adhesion; Cell Line, Tumor; Cell Movement; Cell Proliferation; Coculture Techniques; Colonic Neoplasms; pathology; Fibroblasts; cytology; Humans
- From: Chinese Journal of Gastrointestinal Surgery 2013;16(8):759-763
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of human colon carcinoma-associated fibroblasts (CAFs) on proliferation, adhesion, migration and invasion of colon carcinoma Lovo cells.
METHODSThe co-culture models among colon CAFs, NFs and Lovo cell were established by conditioned medium (CM) of human colon CAFs and colon normal fibroblasts (NFs). Lovo cells in the blank control group was treated with serum-free culture medium. The effects of human colon CAFs on proliferation, adhesion, migration and invasion of colon carcinoma Lovo cells were detected by cell proliferation assay, adhesion assay, migration assay and Transwell invasion assay.
RESULTSAfter co-culture with colon CAFs, the absorbance (A) value of Lovo cells was (0.667±0.059) in 48 h and (0.709±0.030) in 72 h. The A value of Lovo cells adhesion to fibronectin was (0.588±0.067). The cell mobility rates were (35.2±8.7)% in 12 h and (64.6±7.1)% in 24 h. The number of invasive cell was (56.2±4.8). All the above parameters were increased compared with those in the blank control group and NFs group (all P<0.01).
CONCLUSIONHuman colon CAFs can promote the proliferation, adhesion, migration and invasion of colon carcinoma Lovo cells.
