Detection and significance of succinate dehydrogenase of sperm mitochondria.
- Author:
Xiao-Ping XUE
1
;
Xue-Jun SHANG
;
Jian FU
;
Yong-Gang CHEN
;
Yi-Chao SHI
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Case-Control Studies; Cell Survival; Humans; Image Processing, Computer-Assisted; Infertility, Male; enzymology; Male; Mitochondria; enzymology; Sperm Motility; Spermatozoa; enzymology; Succinate Dehydrogenase; metabolism
- From:National Journal of Andrology 2003;9(8):601-603
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect succinate dehydrogenase (SDH) of sperm mitochondria by an improved method, and to evaluate the relationship between SDH and the motility and viability of sperm.
METHODSIn 46 fertile and infertile men (aged from 25 to 36), the motility, viability and mitochondrial SDH of sperm were detected by computer-assisted semen analysis system, dead/live sperm molecular fluorescent probe and an improved cytochemical method. Then, the correlation between the motility and viability of sperm and the positive percentage of sperm mitochondrial SDH were analyzed.
RESULTSIn the 46 fertile and infertile men, the motility and viability of sperm were (67.33 +/- 7.37)% and (79.78 +/- 7.65)%, and the positive percentage of sperm mitochondrial SDH was (74.74 +/- 8.29)%. The motility and viability of sperm and positive percentage of sperm mitochondrial SDH had significant correlation (r = 0.901, P < 0.01; r = 0.876, P < 0.01; r = 0.917, P < 0.01).
CONCLUSIONSDetection of sperm mitochondrial SDH has significance in evaluation of sperm mitochondrial function and may serve as an assisted marker of sperm viability.
