A study of aspermia-related genes by genchips and analysis of the RAP1A gene.

Author: Bo YANG ¹ ; Xiao-Kang GAO ; He WANG ; He-Liang LIU ; Bao-Qi CHEN ; Jian-Lin YUAN ; Guo-Xing SHAO ; Chen SHAO
Author Information

1. Department of Urology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710032, China.
Publication Type:Journal Article
MeSH: Adult; Gene Expression Profiling; Humans; Male; Oligonucleotide Array Sequence Analysis; methods; Oligospermia; genetics; rap1 GTP-Binding Proteins; genetics
From: National Journal of Andrology 2003;9(9):654-657
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo study the differential gene expression profiles between the normal and aspermia human testes by genechips.
METHODSProbes were prepared from mRNA extracted from both normal and aspermia testes and employed on Biostar H-40s genechips to detect the differential gene expression profiles. A distinctly up-regulated gene RAP1A was analyzed by bibliogrphic retrieval.
RESULTSSix hundred and twenty-three differential expressed genes were found, among which the distinctly up-regulated gene RAP1A was closely related to human sperm regulation.
CONCLUSIONSScreening the differential gene expression profiles between the normal and aspermia human testes by genechips can be used in the study of aspermia-related genes.