Protein and mRNA expression of nitric oxide synthase isoforms I and III in rat penile spongium tissues.
- Author:
Jun-Ping SHI
1
;
Yong-Mei ZHAO
;
Gang QI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Blotting, Western; Male; Nitric Oxide Synthase; analysis; genetics; Nitric Oxide Synthase Type I; Nitric Oxide Synthase Type III; Penis; enzymology; RNA, Messenger; analysis; Rats; Rats, Sprague-Dawley; Reverse Transcriptase Polymerase Chain Reaction
- From: National Journal of Andrology 2003;9(9):663-668
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate protein and mRNA expression of nitric oxide synthase (NOS) isoforms I and III in rat penile tissues.
METHODSPenile tissues from male SD rats were investigated. The protein expression of NOS I and III in rat penis was confirmed by immunoprecipitation and Western blot. Gene expression of both NOS I and III was identified by reverse transcription-polymerase chain reaction.
RESULTSThe mRNA and protein products of NOS I and III are both expressed in normal rat penile spongium tissues. But, NOS III mRNA and protein products are more highly expressed than those of NOS.
CONCLUSIONSThese findings support the possibility that penile erection is regulated by different NOS isoforms in rat penile tissues.
