Cloning and sequence analysis of Chlamydia trachomatis heat shock protein 10.
- Author:
Hong-Ye ZHANG
1
;
Jin-Chun LU
Author Information
- Publication Type:Journal Article
- MeSH: Antibodies, Bacterial; blood; Bacterial Proteins; genetics; Base Sequence; Chaperonin 10; chemistry; genetics; immunology; Chlamydia trachomatis; chemistry; immunology; Cloning, Molecular; Female; Humans; Infertility, Female; etiology; Male; Molecular Sequence Data; Polymerase Chain Reaction
- From: National Journal of Andrology 2003;9(9):687-689
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo obtain Chlamydia trachomatis heat shock protein (cHSP) 10 gene from clinical secretion samples.
METHODScHSP10 gene was amplified from 20 cases of clinical secretion samples with positive gold-labelling by specific primers of cHSP10 and identified by sequence analysis.
RESULTScHSP10 full-length gene was amplified from 1 of 20 cases of clinical secretion samples with positive gold-labelling. cHSP10 gene encoding 102 amino acids contains 306 bp, which nuclotide at position 194 changes from T to A, leading to the change of corresponding amino acid.
CONCLUSIONScHSP10 gene may be cloned from clinical secretion samples with positive gold-labelling, which make it possible to further construct expression plasmid of recombinant cHSP10.
