Effect of selection techniques on chromosome and ultrastructure of human spermatozoa.
- Author:
Qiaoyu ZHANG
1
;
Rong QIN
;
Qing CHANG
;
Zhiqing LIANG
;
Changxu SHI
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Chromosome Aberrations; Humans; Male; Spermatozoa; ultrastructure
- From: National Journal of Andrology 2004;10(1):29-31
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo know the effect of different selection techniques on chromosome abnormality rate and the percentage of spermatozoa with relatively intact head ultrastructure in human sperm.
METHODSThe selection techniques of swim-up, Percoll density gradient centrifugation and doule-flection tube were used to observe sperm chromosome abnormality rate, sex chromosomal ratio and the percentage of spermatozoa with relatively intact head ultrastructure.
RESULTSThe sperm chromosome abnormality rate, sex chromosomal ratio and the percentage of spermatozoa with relatively intact head ultrastructure remained unchanged after the application of the three selection techniques(P > 0.05).
CONCLUSIONThe three selection techniques used in this study neither increase the damage to sperm ultrastructure nor exert effects on human chromosome.
