Biological characteristics of female rabbit clitoral smooth muscle cell culture in vitro.

Author: Bo LIU ¹ ; Jihong LIU ; Tao WANG
Author Information

1. Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
Publication Type:Journal Article
MeSH: Animals; Cell Adhesion; Cell Division; Cells, Cultured; Clitoris; cytology; Female; Immunohistochemistry; Myocytes, Smooth Muscle; cytology; Rabbits
From: National Journal of Andrology 2004;10(3):198-201
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo investigate the method of culturing rabbit clitoral smooth muscle cells(SMC) in vitro and their biological characteristics.
METHODSRabbit clitoral SMCs were cultured in vitro by enzymatically dispersed method, and morphological observation, cell counting and immunohistochemical staining were employed to study their biological characteristics.
RESULTSRabbit clitoral SMCs were spindle-shaped and parallelled along their longitudinal axis, and the attachment and proliferation in vitro were rapid.
CONCLUSIONRabbit clitoral SMCs cultured in vitro can grow and maintain their steady characteristics when provided with appropriate culture condition. Clitoral SMCs cultured in vitro can serve as a cell material for researches of clitoris.