Influence of epidermal growth factor and gonadotrophin on the in vitro maturation of human oocytes.
- Author:
Yuan LI
1
;
Zijiang CHEN
;
Lixin ZHAO
;
Mei LI
;
Shuiying MA
;
Li WANG
;
Jingmei HU
Author Information
- Publication Type:Journal Article
- MeSH: Cells, Cultured; Dose-Response Relationship, Drug; Epidermal Growth Factor; pharmacology; Female; Fertilization; drug effects; Gonadotropins; pharmacology; Humans; Oocytes; drug effects; physiology
- From: National Journal of Andrology 2004;10(4):257-262
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of epidermal growth factor(EGF) and different concentrations of gonadotrophin (Gn) on the in vitro maturation of human oocytes.
METHODSEGF was added to the vitro culture medium in order to observe the effect of Gn combined with or without EGF on the result of in vitro maturation. The concentrations of hCG and FSH were changed respectively to observe the difference between the results.
RESULTSAdding EGF to the culture medium improved the maturation rate of oocyte significantly (P < 0.05). There was no difference between the results with different concentrations of hCG and FSH in the culture medium.
CONCLUSIONEGF can improve the results of the in vitro maturation of human oocytes by increasing the maturation rate significantly. Increasing the concentration of Gn does not influence the results of in vitro maturation.
