Fusion expression of Neisseria gonorrhoeae outmembrane protein with a mucosal adjuvant.
- Author:
Jing PAN
1
;
Xuhu MAO
;
Weijun ZHANG
;
Dongshui LU
;
Ping LUO
;
Ning WANG
Author Information
- Publication Type:Journal Article
- MeSH: Bacterial Outer Membrane Proteins; biosynthesis; Bacterial Toxins; biosynthesis; Bacterial Vaccines; immunology; Enterotoxins; biosynthesis; Escherichia coli; genetics; Escherichia coli Proteins; Neisseria gonorrhoeae; chemistry; immunology; Polymerase Chain Reaction; Recombinant Fusion Proteins; biosynthesis; Vaccines, Synthetic; immunology
- From: National Journal of Andrology 2004;10(4):269-274
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo express a fusion protein of Neisseria gonorrhoeae with a mucosal adjuvant.
METHODSThe gene coding Loop VI-VIII(PL678) of porin, an out-membrane protein of Neisseria gonorrhoeae, was obtained by PCR. It was inserted into a plasmid fused with subunit B of heat labile enterotoxin. The recombinant was transformed in E. coli. The expression of fusion protein was analysed by ELISA, SDS-PAGE and Western-blot.
RESULTFusion protein with LTB was successfully expressed, and displayed both the ability of binding GM1 and the reactogenicity with polyclonal antibodies against Neisseria gonorrhoeae.
CONCLUSIONThe expression of fusion protein laid a foundation for the study of the intramolecular vaccine against Neisseria gonorrhoeae.
