A study on the mechanism of bFGF promoting endothelial cells to adhere to polyurethane material.
- Author:
Xulong ZHOU
1
;
Yiping DENG
;
Longchu XIE
Author Information
1. Department of Pathology, Medical Collage of Jinan University, Guangzhou 510632.
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Adhesion;
drug effects;
Cells, Cultured;
Endothelium, Vascular;
cytology;
metabolism;
Fibroblast Growth Factor 2;
pharmacology;
Integrin beta1;
biosynthesis;
Polyurethanes;
Swine
- From:
Journal of Biomedical Engineering
2002;19(3):386-388
- CountryChina
- Language:Chinese
-
Abstract:
bFGF can promote endothelial cell to adhere to artificial polyurethane material. However the mechanism is still unclear. Here the effect of bFGF on endothelial cell expression of integrin beta 1 subunits was determined with Western Blot and image analysis method. The result reveal that the mean gray value of immunostain is 166.1 +/- 9.86 in the experimental group and 175.32 +/- 5.12 in the control group, suggest that bFGF may upregulate the endothelial cell expression of integrin beta 1 subunits. It is concluded that the bFGF may induce the overexpression of endothelial cell integrin beta 1 subunits to increase the cell adhesion onto polyurethane material. It is suggested that bFGF can be taken as an important assistant factor in accomplishment of endothelialization on artificial heart and blood vessel.
