The study of erythrocyte fragility and morphological changes caused by roller pump in vitro.
- Author:
Siwei XU
1
;
Fang CHEN
;
Minjun DING
;
Rukun CHEN
;
Suigen LU
;
Hailin ZHONG
Author Information
1. Department of Cardiovascular Surgery, 2nd Affiliated Hospital, Medical College, Zhejiang University, Hangzhou 310009.
- Publication Type:Journal Article
- MeSH:
Erythrocyte Deformability;
Heart-Lung Machine;
adverse effects;
Hemoglobins;
analysis;
Hemolysis;
Humans;
In Vitro Techniques;
Osmotic Fragility;
Time Factors
- From:
Journal of Biomedical Engineering
2002;19(3):419-422
- CountryChina
- Language:Chinese
-
Abstract:
To observe the erythrocyte fragility and morphological changes of erythrocytes caused by roller pump. Ten tests were divided into two groups, Polystan pediatric pump group A (n = 5) and COBE pump group B (n = 5). Ten whole blood samples (each 400 ml) were circulated in the roller pump for 16 h. Erythrocyte fragility and free hemoglobin were measured before pumping and at every 2 hours during pumping. The possible morphological changes of erythrocytes caused by roller pump were observed by scanning electron microscope. The electron microscopic observation was made before pumping and at every 4 hours throughout pumping. Results showed that the erythrocyte fragility of two groups was not increased during a long period of pumping. The number of acanthocytes of two groups was 1.77/1.81% in the samples before pumping and 6.12/7.13, 9.18/8.73, 13.21/12.89, 16.53/17.21% at 4 h, 8 h, 12 h, and 16 h respectively. The free hemoglobin level of two groups was increased linearly during a long duration of pumping and the index of hemolysis of two groups was 0.296 mg/L/h and 0.3993 mg/L/h respectively. The result shows: 1. the erythrocyte fragility was not increased during a long period of pumping; 2. the erythrocyte membrane was injured or broken by roller pump directly; 3. the morphological changes of erythrocytes would be the basis of post operative hemolysis.
