miR-124a promotes neurite outgrowth by inhibiting iASPP expression.
- Author:
Lifang LIN
1
;
Xi GU
;
Shuhu LIU
;
Xuemin WANG
Author Information
- Publication Type:Journal Article
- MeSH: 3' Untranslated Regions; Gene Expression; Humans; Intracellular Signaling Peptides and Proteins; genetics; metabolism; MicroRNAs; genetics; Neurites; metabolism; Repressor Proteins; genetics; metabolism; Transfection
- From: Journal of Southern Medical University 2014;34(1):31-35
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the role of iASPP as the target gene of miR-124a in neural development.
METHODSUsing the online bioinformatical tool (TargetScan) and by reviewing the relevant studies, we selected iASPP as the candidate target gene of miR-124a involved in early-stage neuronal differentiation. Luciferase reporter assay was used to verify the candidate gene. We transfected M17 cells with a miR-124a overexpression plasmid and detected the changes in the protein expression of iASPP using Western blotting. With retinoic acid-induced M17 cells as the neuronal differentiation model, the role of iASPP in early-stage neuronal differentiation was investigated by gene overexpression and gene interference techniques.
RESULTSmiR-124a inhibited the expression of iASPP in M17 cells by interacting with the 3'UTR of iASPP gene. miR-124a promoted neurite outgrowth of the cells, which was blocked by iASPP overexpression.
CONCLUSIONmiR-124a promotes neurite outgrowth of M17 cells by inhibiting iASPP expression.
