Overexpression of Wnt3 inhibits apoptosis of hepatic progenitor cells in vitro.
- Author:
Xifeng ZHANG
1
;
Daixi HU
;
Chao ZHANG
;
Qin ZHONG
;
Tao FENG
;
Jiayi HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Cells, Cultured; Genetic Vectors; Hepatocytes; cytology; Humans; Proto-Oncogene Proteins c-bcl-2; metabolism; Stem Cells; cytology; Transfection; Wnt3 Protein; metabolism; bcl-X Protein; metabolism
- From: Journal of Southern Medical University 2014;34(1):46-50
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of adenoviral vector-mediated over-expression of Wnt3 on the apoptosis of hepatic progenitor cells in vitro.
METHODSHepatic progenitor cells transfected with Ad-GFP-Wnt3 vector or the control vector Ad-GFP were examined for cell apoptosis under fluorescence microscopy with Hoechst 33342 staining, and the proportion of apoptotic cells were determined by flow cytometric analysis with Annexin-PE/7-ADD staining. The mRNA and protein expressions of Bax, Bcl-2 and Bcl-xl in the cells were detected by real-time PCR and Western blotting, respectively.
RESULTSReal-time PCR and Western blotting showed a high expression of Wnt3 in Ad-GFP-Wnt3-transfected hepatic progenitor cells, which exhibited significantly decreased cell apoptosis as compared with the control group. The expressions of Bcl-2 and Bcl-xl mRNA and proteins increased significantly while Bax expression decreased obviously in Ad-GFP-Wnt3-transfected cells (P<0.05).
CONCLUSIONSAdenoviral vector-mediated over-expression of Wnt3 can suppress apoptosis of hepatic progenitor cells possibly through the Bcl-2 pathway.
