miR-520a regulates ErbB4 expression and suppresses proliferation and invasion of esophageal squamous cell carcinoma.
- Author:
Wenguang YE
1
;
Qinglin YAO
;
Mingxin ZHANG
;
Qinsheng WEN
;
Jingjie WANG
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Squamous Cell; metabolism; pathology; Cell Line, Tumor; Cell Movement; Cell Proliferation; Esophageal Neoplasms; metabolism; pathology; Gene Expression Regulation, Neoplastic; Humans; MicroRNAs; metabolism; Receptor, ErbB-4; metabolism
- From: Journal of Southern Medical University 2014;34(2):164-168
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the role of miR-520a in regulation ErbB4 expression and the biological behavior of esophageal squamous cell carcinoma (ESCC).
METHODSThe role of miR-520a in regulating the expression of ErbB4 was investigated by Western blotting and luciferase reporter assay system. The effect of miR-520a on the proliferation and invasion of ESCC cells was detected by MTT and Transwell invasion assay, respectively.
RESULTSWestern blotting and luciferase reporter assay revealed that miR-520a down-regulated the expression of ErbB4 in vitro. miR-520a significantly inhibited the proliferation and suppressed the invasion of ESCC cell line Eca109.
CONCLUSIONmiR-520a regulates the expression of ErbB4 and suppresses the proliferation and invasion of ESCC cells in vitro, suggesting its role as a tumor suppressor.
