2-Deoxy-D-glucose combined with Taxol inhibits VEGF expression and induces apoptosis in orthotopically transplanted breast cancer in C3H mice.
- Author:
Qianwen ZHANG
1
;
Huaiyong GAN
;
Zenong CHENG
;
Surong ZHAO
;
Chao CHEN
;
Chenchen JIANG
;
Hao LIU
;
Zhiwen JIANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antineoplastic Agents; pharmacology; therapeutic use; Apoptosis; Breast Neoplasms; drug therapy; pathology; Cell Line, Tumor; Deoxyglucose; pharmacology; therapeutic use; Drug Synergism; Female; Mice; Mice, Inbred C3H; Paclitaxel; pharmacology; therapeutic use; Vascular Endothelial Growth Factor A; metabolism; Xenograft Model Antitumor Assays
- From: Journal of Southern Medical University 2014;34(2):193-196
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the antineoplastic effects of 2-Deoxy-D-glucose (2-DG) combined with Taxol on orthotopically transplanted breast cancer in C3H mice and explore the mechanism.
METHODSC3H mice bearing orthotopically transplanted breast cancer xenograft were randomly divided into 4 groups, namely the control group, 2-DG group, Taxol group, and 2-DG+Taxol group. The corresponding drugs were administered intraperitoneally every 3 days for 18 consecutive days, and the tumor volume was measured every 3 days to draw the tumor growth curve. The mice were then sacrificed to measure the tumor weight on day 19 and examine tumor cell apoptosis with TUNEL assay and VEGF expression using immunohistochemistry.
RESULTS2-DG combined with Taxol obviously suppressed the tumor growth with a tumor inhibition rate of 66.06% as compared to the rate of 36.97% in Taxol group. The combined treatment also caused more obvious cell apoptosis and significantly reduced VEGF expression in the tumor cells as compared with the other groups.
CONCLUSION2-DG can enhance the inhibitory effect of Taxol on orthotopically transplanted breast cancer xenograft in C3H mice probably by inducing tumor cell apoptosis and lowering VEGF expressions.
