miR-221 mediates epithelial-mesenchymal transition-related gene expressions via regulation of PTEN/Akt signaling in drug-resistant glioma cells.
- Author:
Qiang XIE
1
;
Zuoping HUANG
;
Yongrong YAN
;
Feng LI
;
Xueyun ZHONG
Author Information
- Publication Type:Journal Article
- MeSH: Cadherins; metabolism; Cell Line, Tumor; Drug Resistance, Neoplasm; Epithelial-Mesenchymal Transition; Gene Expression Regulation, Neoplastic; Humans; MicroRNAs; genetics; metabolism; Multidrug Resistance-Associated Proteins; metabolism; PTEN Phosphohydrolase; metabolism; Proto-Oncogene Proteins c-akt; metabolism; Signal Transduction; Vimentin; metabolism
- From: Journal of Southern Medical University 2014;34(2):218-222
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the correlation between miR-221 and epithelial-mesenchymal transition (EMT) in drug-resistant glioma cells.
METHODSThe expression levels of miR-221, PTEN, p-Akt, E-cadherin, vimentin, and MRP1 were quantitatively analyzed in Z1 cells (primary drug-resistant cells), Z2 cells (drug-sensitive cells) and Z2-BCNU cells (drug-resistant cells) using fluorescent real-time PCR and Western blotting.
RESULTSThe expression levels of PTEN were significantly increased in Z2 cells compared with Z1 and Z2-BCNU cells which overexpressed miR-221 and vimentin. The expression levels of vimentin, p-Akt and MRP1 were significantly decreased in Z2 cells overexpressing E-cadherin.
CONCLUSIONMiR-221 regulates the expression of EMT-related genes through down-regulation of PTEN and activation of PI3-K/Akt signaling.
