Peperphentonamine hydrochloride protects against gentamicin-induced cochlea damage by lowering cochlear caspase-3 expression in guinea pigs.

Hao Chen; Minqiang Xie; Jian WU; Wei LI; Yonghe LI

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Peperphentonamine hydrochloride protects against gentamicin-induced cochlea damage by lowering cochlear caspase-3 expression in guinea pigs.

Author: Hao CHEN ¹ ; Minqiang XIE ; Jian WU ; Wei LI ; Yonghe LI
Author Information

1. Department of Otolaryngology and Head and Neck Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. E-mail: kitty2chh@aliyun.com.
Publication Type:Journal Article
MeSH: 3,4-Methylenedioxyamphetamine; analogs & derivatives; pharmacology; Animals; Apoptosis; drug effects; Caspase 3; metabolism; Cochlea; drug effects; pathology; Evoked Potentials, Auditory, Brain Stem; Gentamicins; adverse effects; Guinea Pigs
From: Journal of Southern Medical University 2014;34(3):329-334
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo study the protective effect of peperphentonamine hydrochloride (PPTA) against gentamicin-induced cochlear damage and its mechanism to inhibit cell apoptosis.
METHODSGuinea pigs with normal hearing were randomized into control, gentamicin, and PPTA treatment groups, and the guinea pigs models of gentamicin-induced cochlear damage received intraperitoneal injection of PPTA. The changes of hearing of the guinea pigs were evaluated with auditory brainstem response (ABR) test, and the protein expression of caspase-3 in the cochlear tissue was detected using Western blotting. TUNEL staining, scanning and transmission electron microscopy were performed to observe the morphological changes of the cochlea.
RESULTSThe threshold in ABR in PPTA treatment group was significantly higher than that in the control group (P<0.05) but significantly lower than that in gentamicin group. Western blotting showed a significantly increased caspase-3 expression in gentamicin group (P<0.001); caspase-3 expression in PPTA group was obviously higher than that in the control group but much lower than that in gentamicin group (P<0.001). TUNEL assay and electron microscopy revealed serious damages of the hair cells in gentamicin group with numerous apoptotic cells in the organ of Corti, stria vascularis and spiral ganglion, and such cochlear damages were obviously alleviated in PPTA group.
CONCLUSIONPPTA can protect against gentamicin-induced cochlear damage in guinea pigs by decreasing the protein expression of caspase-3 to inhibit cell apoptosis.