Effect of interleukin-1β on expressions of activin A and its related factors in cultured endometrial stromal cells from patients with endometriosis.
- Author:
Lei LI
1
;
Mubiao LIU
;
Fengcheng ZHU
;
Ying PAN
Author Information
- Publication Type:Journal Article
- MeSH: Activins; metabolism; Cells, Cultured; Endometriosis; metabolism; Endometrium; cytology; Female; Humans; Interleukin-1beta; pharmacology; Stromal Cells; drug effects; metabolism
- From: Journal of Southern Medical University 2014;34(3):410-413
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of interleukin-1β (IL-1β) on the expressions activin A, follistatin, and cripto in cultured human endometrial stromal cells (HESCs) form patients with endometriosis.
METHODSCultured HESCs were stimulated with 250, 500, and 750pg/ml IL-1β, and the mRNA and protein expressions of activin A, follistatin, and cripto were assayed using real-time reverse transcriptase-polymerase chain reaction and enzyme-linked immunosorbent assay.
RESULTSIL-1β treatment caused significant dose-dependent increments of the mRNA and protein expressions of activin A and follistatin and of the mRNA expression of cripto in cultured HESCs.
CONCLUSIONIL-1β can affect the expressions of activin A, follistatin and cripto in HESCs from patients with endometriosis.
