Effect of antibacterial peptide LL-37 on the integrity of Acinetobacter baumannii biofilm.
- Author:
Pengwei SHI
1
;
Yanbin GAO
;
Zhiyang LU
;
Lei YANG
Author Information
- Publication Type:Journal Article
- MeSH: Acinetobacter baumannii; drug effects; physiology; Biofilms; drug effects; Cathelicidins; pharmacology; Microbial Sensitivity Tests
- From: Journal of Southern Medical University 2014;34(3):426-429
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of antibacterial peptide LL-37 on the integrity of Acinetobacter baumannii biofilm.
METHODSA model of Acinetobacter baumannii biofilm in vitro was constructed by plates and crystal violet staining method, and the minimal inhibitory concentration of LL-37 was determined by broth dilution. The biofilm morphology was observed by scanning electron microscopy and biofilm formation was analyzed by the crystal violet staining of the adherent biofilm in the presence of antibacterial peptide LL-37.
RESULTSIn the Acinetobacter baumannii biofilm model, the minimal inhibitory concentration of LL-37 was 64 µg/ml; LL-37 caused structural damage of the biofilm at a low concentration of 2.5 µg/ml. The biofilm was decreased gradually as the concentration of LL-37 increased.
CONCLUSIONLL-37 even at a concentration far below its minimal inhibitory concentration can cause structural damage of Acinetobacter baumannii biofilm in vitro.
