Changes in gap junctional intercellular communication in rabbits lens epithelial cells induced by low power density microwave radiation.
- Author:
Juan YE
1
;
Ke YAO
;
Qunli ZENG
;
Deqiang LU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cataract; etiology; Cell Communication; radiation effects; Connexin 43; analysis; Epithelial Cells; radiation effects; Fluorescent Antibody Technique, Indirect; Gap Junctions; radiation effects; Lens, Crystalline; radiation effects; Microwaves; adverse effects; Rabbits
- From: Chinese Medical Journal 2002;115(12):1873-1876
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo demonstrate the changes in gap junctional intercellular communication (GJIC) mediated by low power density microwave radiation in rabbits lens epithelial cells (LECs) and its mechanisms.
METHODSRabbits' eyes were exposed to 5 mW/cm(2) and 10 mW/cm(2) power densities of microwave radiation for 3 hours. The fluorescence-recovery-after-photobleaching (FRAP) method was used to determine the GJIC. The localization and function of connexin 43 in LECs was detected by laser scanning confocal microscopy.
RESULTSThe GJIC of rabbits LECs was inhibited by microwave radiation especially in the 10 mW/cm(2) irradiated samples. A decrease in connexin 43-positive staining was seen in 5 mW/cm(2) x 3 h treated LECs. Intracellular space accumulation and cytoplasmic internalization were clearly demonstrated in 10 mW/cm(2) group.
CONCLUSIONSLow power densities microwave radiation (5 mW/cm(2) and 10 mW/cm(2)) induces damage to connexin 43 and inhibits the GJIC of rabbits LECs. These changes result in an osmotic imbalance within the lens and induce early cataract. 5 mW/cm(2) or 10 mW/cm(2) microwave radiation is cataractogenic.
