Genotype identification of Orientia tsutsugamushi isolated from Nan Peng Lie Islands in China.
- Author:
Guifu PENG
1
;
Zhibing WANG
;
Shanshan WANG
;
Jialiang HUANG
;
Pulin JIANG
;
Nianhua ZENG
;
Jinhua LIU
;
Shaofan ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; China; DNA, Bacterial; analysis; Genotype; Mice; Orientia tsutsugamushi; genetics; Polymorphism, Restriction Fragment Length; Rats
- From: Chinese Medical Journal 2002;115(12):1881-1882
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo identify genotype of eight strains of Orientia tsutsugamushi (O. tsutsugamushi) isolated from Nan Peng Lie Islands in China and establish tsutsugamushi disease nature foci for this region.
METHODSThe nested polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) were used. Three primers were selected from the DNA sequence of the gene encoding type-specific 56-kDa protein of the Karp strain. The positive products were digested by Hine II and Pst I, meanwhile profiles specific to each strain were analyzed.
RESULTSThree genotypes of O. tsutsugamushi including Karp, Kato and a new strain existed on Nan Peng Lie Islands.
CONCLUSIONNan Peng Lie Islands is tsutsugamushi disease nature foci.
