Homocysteine alters monocyte-endothelial interaction in vitro.
- Author:
Xuewei GUO
1
;
Nicholas Peter DUDMAN
Author Information
- Publication Type:Journal Article
- MeSH: Arteriosclerosis; etiology; Cell Adhesion; drug effects; Cell Communication; drug effects; Cell Movement; drug effects; Dose-Response Relationship, Drug; Endothelium, Vascular; cytology; drug effects; Homocysteine; pharmacology; Humans; Monocytes; drug effects; physiology
- From: Chinese Medical Journal 2003;116(1):34-38
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo determine whether homocysteine induced endothelial damage through monocyte-endothelial interaction and to characterize both cell types in vitro.
METHODSRadiomethods were performed on monocyte adhesion to/through endothelium and endothelial damage experiments.
RESULTSHomocysteine-treated endothelial cells increased monocyte adhesion and transmigration. Homocysteine-treated monocytes induced endothelial detachment, but this effect was blocked by catalase. These effects were increased with higher concentrations of homocysteine. Monocyte surface glycoprotein antibodies CD11b/CD18 and CD14 inhibited these processes.
CONCLUSIONSHomocysteine alters monocyte-endothelial interaction in vitro, eventually bringing about endothelial damage through release of H(2)O(2). These phenomena are mediated through monocyte surface glycoproteins CD11b/CD18 and CD14. Upregulation of these processes in vivo may contribute to acceleration of atherosclerosis in patients with elevated plasma homocysteine levels.
