Effects of red orpiment on cell morphology and expression of PML mRNA and protein in NB4 and HL-60 cells.
- Author:
Lu ZHONG
1
;
Fangyuan CHEN
;
Jieying HAN
;
Nianxian SHAO
;
Renrong OUYANG
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents; pharmacology; Apoptosis; drug effects; Arsenicals; pharmacology; HL-60 Cells; Humans; Leukemia, Promyelocytic, Acute; drug therapy; metabolism; pathology; Medicine, Chinese Traditional; Neoplasm Proteins; analysis; genetics; Nuclear Proteins; Promyelocytic Leukemia Protein; RNA, Messenger; analysis; Transcription Factors; analysis; genetics; Tumor Cells, Cultured; Tumor Suppressor Proteins
- From: Chinese Medical Journal 2003;116(1):148-150
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the effects of red orpiment on cell morphology, expression of promyelocytic leukemia (PML) mRNA and its protein localization in NB4 and HL-60 cell lines.
METHODSCell morphology was assayed by Wright's staining and fluorescence staining, while PML mRNA expression was determined by RT-PCR. PML protein localization by evaluated by immunofluorescence staining.
RESULTSThe typical apoptosis was found in NB4 and HL-60 cells after treatment with red orpiment. The fusion protein was no longer observed in NB4 cells, PML protein was relocated, and then degraded. In HL-60 cells, PML protein underwent a similar progress. The expression of promyelocytic leukemia (PML) mRNA was not changed in the treated cells.
CONCLUSIONRed orpiment inhibits the proliferation of leukemia cells by inducing them to undergo apoptosis.
