Cloning the full-length cDNA of a gene responsible for vascular smooth muscle cell proliferation in atherogenesis and study of function.
- Author:
Gaofeng ZHAO
1
;
Peng GAO
;
Hua ZHANG
;
Liming LOU
;
Huihong QIAO
;
Mingpeng SHE
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acid Sequence; Arteriosclerosis; genetics; Base Sequence; Blotting, Northern; Cell Division; drug effects; Cells, Cultured; Cloning, Molecular; Gene Library; Humans; Lipoproteins, LDL; pharmacology; Molecular Sequence Data; Muscle, Smooth, Vascular; cytology; drug effects; Nucleic Acid Hybridization
- From: Chinese Medical Journal 2003;116(2):166-170
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo clone the full-length cDNA of a gene responsible for vascular smooth muscle cell (v-SMC) proliferation in atherogenesis, and study its function.
METHODSOxidized low density lipoprotein (ox-LDL) at optimal concentration was used as the stimulant to induce v-SMC proliferation in culture medium. A cDNA subtractive library of v-SMC proliferation specific to ox-LDL stimulation was established using subtractive hybridization technique. Methods, including blotting, Northern hybridization and gene sequencing, were used to clone new gene fragments. By using full-length cDNA screening and protein expression techniques, one full-length cDNA was cloned and its function was studied.
RESULTSOne full-length cDNA was cloned. The new gene (Genbank AF 174647) expressed a 44 kDa protein, which might be associated with the activity of ox-LDL.
CONCLUSIONThe new gene cloned may be associated with SMC proliferation in atherogenesis.
