Black soybean anthocyanins attenuate inflammatory responses by suppressing reactive oxygen species production and mitogen activated protein kinases signaling in lipopolysaccharide-stimulated macrophages.
10.4162/nrp.2017.11.5.357
- Author:
Jin Nam KIM
1
;
Sung Nim HAN
;
Tae Joung HA
;
Hye Kyeong KIM
Author Information
1. Department of Food Science and Nutrition, The Catholic University of Korea, 43 Jibong-ro, Wonmi-gu, Bucheon-si, Gyeonngi 14662, Korea. hkyeong@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
Anthocyanins;
anti-inflammatory agents;
macrophage;
mitogen-activated protein kinases;
reactive oxygen species
- MeSH:
Anthocyanins*;
Anti-Inflammatory Agents;
Cyclooxygenase 2;
Cytokines;
Inflammation;
Interleukin-6;
JNK Mitogen-Activated Protein Kinases;
Macrophages*;
Mitogen-Activated Protein Kinases*;
Nitric Oxide;
Nitric Oxide Synthase;
Oxidative Stress;
Phosphorylation;
Phosphotransferases;
RAW 264.7 Cells;
Reactive Oxygen Species*;
Soybeans*;
Tumor Necrosis Factor-alpha
- From:Nutrition Research and Practice
2017;11(5):357-364
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND/OBJECTIVES: Oxidative stress is closely related with inflammation and development of many diseases. Black soybean seed coat contains high amount of anthocyanins, which are well-known for free radical scavenging activities. This study investigated inflammatory response and action mechanism of black soybean anthocyanins with regard to antioxidant activity in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. MATERIALS/METHODS: RAW 264.7 cells were treated with anthocyanins extracted from black soybean seed coats in a concentration range of 12.5 to 100 µg/mL. The production of reactive oxygen species (ROS), secretion of pro-inflammatory mediators and cytokines, and the signaling in the mitogen activated protein kinases (MAPKs) pathway were examined. RESULTS: Black soybean anthocyanins significantly decreased LPS-stimulated production of ROS, inflammatory mediators such as nitric oxide (NO) and prostaglandin E₂, and pro-inflammatory cytokines, including tumor necrosis factor α and interleukin-6, in a dose-dependent manner without cytotoxicity (P < 0.001). Black soybean anthocyanins downregulated the expression of inducible NO synthase and cyclooxygenase-2 in LPS-stimulated RAW 264.7 cells (P < 0.001). Moreover, black soybean anthocyanins inhibited LPS-induced phosphorylation of MAPKs, including extracellular signal-regulated kinase, c-Jun N-terminal kinase, and p38 (P < 0.001). CONCLUSION: These results suggest that black soybean anthocyanins exert anti-inflammatory activity by inhibiting ROS generation and subsequent MAPKs signaling, thereby inhibiting inflammatory responses.
