Local tissue hypoxia and formation of nasal polyps.
- Author:
Shu JIANG
1
;
Zhen DONG
;
Dongdong ZHU
;
Zhanquan YANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Hypoxia; physiology; Cells, Cultured; Endothelial Growth Factors; genetics; Enzyme-Linked Immunosorbent Assay; Erythropoietin; genetics; Humans; Intercellular Signaling Peptides and Proteins; genetics; Interleukin-1; pharmacology; Lymphokines; genetics; Nasal Mucosa; metabolism; Nasal Polyps; etiology; metabolism; RNA, Messenger; analysis; Tumor Necrosis Factor-alpha; pharmacology; Vascular Endothelial Growth Factor A; Vascular Endothelial Growth Factors
- From: Chinese Medical Journal 2003;116(2):243-247
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo explore the response of nasal mucosa epithelial cells to hypoxia in terms of formation of nasal polyps (NP).
METHODSEpithelial cells of NP and inferior turbinate (IT) were cultured serum-free under normal oxygen and hypoxic circumstances with stimulation of IL-1 beta and TNF alpha. The vascular endothelial growth factor (VEGF) mRNA and VEGF protein levels of the cultured cells were detected using in situ hybridization and ELISA, respectively.
RESULTSThe expression of VEGF mRNA was significantly higher in epithelial cells of NP than in IT exposed to pro-inflammatory cytokines or hypoxia (P < 0.01). VEGF levels were higher in NP epithelial cells than those of IT (P < 0.01) under hypoxia.
CONCLUSIONVEGF-induced by hypoxia is very important for the early stages of forming polyps.
