Determnination of betaine in Fufang Guilu granule by HPLC.
- Author:
Ming-Wang XU
1
;
Xiu-Zhi YUAN
;
Yan-Weng LIU
;
Xu-Ju SHEN
Author Information
- Publication Type:Journal Article
- MeSH: Betaine; analysis; Chromatography, High Pressure Liquid; methods; Codonopsis; chemistry; Drug Combinations; Drugs, Chinese Herbal; chemistry; Lycium; chemistry; Plants, Medicinal; chemistry; Quality Control; Reproducibility of Results
- From: China Journal of Chinese Materia Medica 2006;31(6):462-464
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo describe a HPLC method for assessing betaine in Fufang Guilu granule.
METHODThe content of betainephenaxcyl bromide in Fufang Guilu granule was determined by HPLC. The analytical column was a shim-pack CLC-ODS (6.0 mm x 150 mm) filling a 5 microm stationary phase; The mobile phase consisted of acetonitrile-water(35:65) with 0.1 mol x L(-1) NaClO4; The flow-rate was 1 mL x min (-1); The detector was set at 254 nm.
RESULTThe calibration curve was linear over the range of 0.09-0.585 microg (r = 0.9997). The average recovery of the method was 98.4%, RSD 2.5% (n = 5).
CONCLUSIONThe results showed that this method was reliable and accurate, and can be used for quality control of Fufang Guilu granule.
