Effect of Herba Epimedii flavone on the osteoblasts metabolism in vitro.
- Author:
Yi-Heng LIU
1
;
Hai-Ying ZHANG
;
Hong-Min ZANG
;
Jun-Chang CHENG
Author Information
- Publication Type:Journal Article
- MeSH: Alkaline Phosphatase; metabolism; Animals; Animals, Newborn; Carrier Proteins; biosynthesis; genetics; Cell Proliferation; drug effects; Cells, Cultured; Epimedium; chemistry; Flavones; isolation & purification; pharmacology; Glycoproteins; biosynthesis; genetics; Membrane Glycoproteins; biosynthesis; genetics; Osteoblasts; cytology; metabolism; Osteoprotegerin; Plants, Medicinal; chemistry; RANK Ligand; RNA, Messenger; biosynthesis; genetics; Rats; Rats, Sprague-Dawley; Receptors, Cytoplasmic and Nuclear; biosynthesis; genetics; Receptors, Tumor Necrosis Factor; biosynthesis; genetics
- From: China Journal of Chinese Materia Medica 2006;31(6):487-490
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effect of Herba Epimedii flavone (HEF) on the osteoblast metabolism in vitro.
METHODOsteoblast were obtained from new born rat calvaria by digestive enzymes. MTF, PNPP and RT-PCR were used to observe the proliferation, activity of ALP and mRNA expression of OPG and RANKL of cultured osteoblasts in vitro.
RESULTIt was found that HEF had the effect on stimulating cell proliferation, activity of ALP and the mRNA expression of OPG of cultured osteoblasts (P < 0.01, P < 0.05).
CONCLUSIONHEF can promote the proliferation, the differentiation and the expression of OPG mRNA of the osteoblasts cultured in vitro.
