Puerarin suppresses the proliferation of vscular smooth muscule cells and c-fos and bcl-2 protein expression.
- Author:
Yi-Zhou XU
1
;
Yan GAO
;
Pei-Zhang LI
;
Ning-Fu WANG
;
Hai-Ying XU
;
Guo-Xin TONG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Aorta, Thoracic; cytology; Cell Proliferation; drug effects; Isoflavones; isolation & purification; pharmacology; Muscle, Smooth, Vascular; cytology; Myocytes, Smooth Muscle; cytology; metabolism; Plants, Medicinal; chemistry; Proto-Oncogene Proteins c-bcl-2; metabolism; Proto-Oncogene Proteins c-fos; metabolism; Pueraria; chemistry; Rats; Rats, Sprague-Dawley; Thrombin; antagonists & inhibitors; Vasodilator Agents; pharmacology
- From: China Journal of Chinese Materia Medica 2006;31(6):490-493
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the role of puerarin on the proliferation of vascular smooth muscle cells(VSMC) induced by thrombin (T) and the effect of puerarin on the c-fos and bcl-2 protein expression.
METHODCell number and cell cycle analysis using flow cytometry were adopted as two different indicators of effects on proliferation of VSMC. Western blot was used to indicate the changes of c-fos and bcl-2 protein after 24 h of treatment of T and puerarin.
RESULT1.5 x 10(-5) - 1.5 x 10(-3) mol x L(-1) puerarin could significantly suppress this stimulation of VSMC proliferation and DNA synthesis induced by T. Western blot demonstrated that after 24 hour of treatment with T and puerarin, T could significantly increase c-fos and bcl-2 protein and 1.5 x 10(-5) - 1.5 x 10(-3) mol x L(-1) puerain could significantly suppress this increase.
CONCLUSIONpuerarin can suppress the proliferation and DNA synthesis of VSMC promoted by T. This inhibitory effects of puerarin are closely related with the suppression of c-fos and bcl-2 protein.
