Quality standard for Xindi soft capsule.
- Author:
Yu-Jie WANG
1
;
Yi ZHANG
;
Rui GU
;
Xian-Rong LAI
;
Ping WANG
;
Zhu-Jun ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Capsules; Chromatography, Thin Layer; Drugs, Chinese Herbal; administration & dosage; chemistry; isolation & purification; standards; Flavonols; analysis; Hippophae; chemistry; Kaempferols; analysis; Plants, Medicinal; chemistry; Quality Control; Quercetin; analysis; Reproducibility of Results
- From: China Journal of Chinese Materia Medica 2006;31(7):552-597
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish the quality control standard of Xindi soft capsule.
METHODQuercetin, kaempferol and isorhamnetin were isolated by TLC with chloroform-ethyl formate-formic acid (5:4:1). The chromatographic separation was performed on a Diamonsil C18 column (4.6 mm x 250 mm, 5 microm). Acetonitrile-water-phosphoric (30:70:0.1) as mobile phase. The flow rate was 1 mL x min(-1) and column temperature was set at 40 degrees C. The UV detection wavelength was set at 254 nm.
RESULTQuercetin, Kaempferol and Isorhamnetin could be identified by TLC. Quercetin showed a good linear relationship at a range of 0.412-1.648 microg, r = 0.999 9, the average recovery was 96.8%, and RSD was 0.9% (n = 6). Kaempferol showed a good linear relationship at a range of 0.021-0.083 microg, r = 0.999 8, the average recovery was 96.9%, and RSD was 2.0% (n = 6). Isorhamnetin showed a good linear relationship at a range of 0.183-0.732 microg, r = 0.999 9, the average recovery was 97.1%, and RSD was 1.6% (n = 6).
CONCLUSIONThe method is accurate with the good reproducibility and can be used for the quality control of Xindi soft capsule.
