Search for Differentially Expressed Genes in Preterm Human Placentae and Their Membranes by Annealing Control Primer System.
- Author:
Hyun Sun KO
1
;
Yeun Hee KIM
;
Ki Cheol KIL
;
Ju Hee JEONG
;
Dong Eun YANG
;
Dae Young CHUNG
;
Tai Churl PARK
;
Soo Pyung KIM
;
Jong Chul SHIN
Author Information
1. Department of Obstetrics and Gynecology, College of Medicine, Catholic University of Korea, Seoul, Korea. cshin@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
Preterm;
GeneFishing;
Annealing control primers (ACPs)
- MeSH:
Acyl-CoA Dehydrogenase;
Amnion;
Chorion;
DNA, Mitochondrial;
Down-Regulation;
Female;
Heparin;
Humans*;
Insulin-Like Growth Factor II;
Membranes*;
Obstetric Labor, Premature;
Placenta*;
Pregnancy;
Proteoglycans;
Ribosomal Proteins;
RNA;
Tissue Inhibitor of Metalloproteinase-1;
Transcriptome;
Up-Regulation
- From:Korean Journal of Perinatology
2005;16(4):300-308
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: The aim of this study was to investigate the gene expression profiles using GeneFishingTM kit in human placentae and their membranes delivered at preterm caused by preterm labor. METHODS: Specimens were obtained from placenta, chorion, and amnion delivered at preterm and term, respectively. Total RNAs were isolated from each specimen. Thereafter, the profiles of expression genes between preterm and term specimens were compared using a new and accurate reverse transcription-polymerase chain reaction (RT-PCR) that involves annealing control primers (ACPs) to identify the genes expressed differentially and screened by basic local alignment search tool (BLAST) search. RESULTS: Using 20 ACPs, 13 differentially expressed genes (DEGs) were identified and sequenced. 7 of them were expressed up-regulation, while 6 were expressed down-regulation in preterm deliveries. A BLAST searches revealed that 11 were known genes and 2 were unknown genes. Among known genes, up-regulated genes were insulin-like growth factor II associated protein, vigilin, acyl-Coenzyme A dehydrogenase, tissue inhibitor of metalloproteinase 1 (TIMP1), ribosomal protein S26 (RPS26), follistatin-like 1 (FSTL1) and down-regulated genes were two mitochondrial DNAs, ribosomal protein S28 (RPS28), transglutaminase 2 (TGM2), heparin sulfate proteoglycan (HSPG, perlecan). CONCLUSION: This study shows that the ACP system is a good method for the identification of preterm-related genes. Furthermore, this study suggests that further analysis of the differentially expressed genes in preterm we have identified should provide insights into the molecular basis of preterm delivery caused by preterm labor.
