Study on purification of 2,3,5,4'-tetrahydroxy-stilbene-2-O-beta-D-glycoside from Polygonum multiflori.
- Author:
Tao-tao JIANG
1
;
Zhen OUYANG
;
Ya-dong MIAO
;
Xiao-feng ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Absorption; Glycosides; chemistry; isolation & purification; Plant Extracts; chemistry; isolation & purification; Plants, Medicinal; chemistry; Polygonum; chemistry; Resins, Synthetic; chemistry; Stilbenes; chemistry; isolation & purification
- From: China Journal of Chinese Materia Medica 2006;31(8):646-649
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the conditions and parameters of purifying 2,3,5,4'-tetrahydroxy-stilbene-2-O-beta-D-glycoside from Polygonum multiflori.
METHODAbsorption capacity of four resins for 2,3,5,4'-tetrahydroxy-stilbene-2-O-beta-D-glycoside was compared. With the adsorption ability as indexes, the process of absorbing and purifying 2,3,5,4'-tetrahydroxy-stilbene-2-O-beta-D-glycoside from P. multiflori with S-8 macroporous resin absorbent was selected by orthogonal design.
RESULTThe S-8 resin was the best of the four resins. The optimum process condition was 50% ethanol as eluting solvent, the flow rate at 1.5 mL x min(-1), pH at 7-8, and the solution concentration at 0.2 g x mL(-1). The absorption capacity by this process was 36.89 mg x g(-1).
CONCLUSIONThe process is simple and convenient and the regeneration of resin is easy, so this method of purification is advisable.
