Effect of delayed preconditioning induced by Sini decoction on myocardial cell apoptosis and its mitochondrial mechanism.
- Author:
Ying LIU
1
;
Wei-kang WU
;
Chen CHEN
;
Xin-feng DUAN
Author Information
- Publication Type:Journal Article
- MeSH: Aconitum; chemistry; Animals; Apoptosis; drug effects; Caspase 3; metabolism; Cytochromes c; metabolism; Drug Combinations; Drugs, Chinese Herbal; isolation & purification; pharmacology; Ginger; chemistry; Glycyrrhiza uralensis; chemistry; Ischemic Preconditioning, Myocardial; methods; Male; Mitochondria, Heart; metabolism; Myocardial Reperfusion Injury; metabolism; pathology; Myocytes, Cardiac; pathology; Plants, Medicinal; chemistry; Random Allocation; Rats; Rats, Sprague-Dawley; bcl-X Protein; metabolism
- From: China Journal of Chinese Materia Medica 2006;31(15):1269-1272
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the mechanism of myocardial cell apoptosis during delayed preconditioning induced by Sini decoction (SND).
METHODSD rats were divide into four groups: control, sham, I/R and SND groups. The rats in I/R group, left anterior descending coronary artery (LAD) was occluded for 1h and reperfused for 1 h. The rats in SND group were pretreated with Sini decoction (5 g x kg(-1) x d(-1)) for three days, the last treatment was pretreated 24 h before the index occlusion. Cell apoptosis was measured by flow cytometry, cytochrome C and bcl-xl were detected by Western blotting and the activity of caspase-3 was detected by assay kit.
RESULTAs compared with I/R group, apoptosis rate of myocardial cell, the release of cytochrome C from mitochondria and the activity of caspase-3 were significantly decreased, and the expression of bcl-xl protein was elevated in SND group.
CONCLUSIONThe delayed preconditioning induced by Sini decoction decreased myocardial cell apoptosis. The mechanism may be related to the inhibition of mitochondria signal pathway of apoptosis.
