Mechanism of Shenqi compound recipe anti-earlier diabetic artherosclerosis in GK rats.
- Author:
Hong-min ZHANG
1
;
Shi-wei CHEN
;
Chun-guang XIE
;
Yi-qiang XIE
;
Xi-fang DENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Aorta; metabolism; Astragalus membranaceus; chemistry; Atherosclerosis; etiology; metabolism; Chemokine CCL2; biosynthesis; blood; genetics; Diabetes Mellitus, Type 2; complications; metabolism; Drug Combinations; Drugs, Chinese Herbal; isolation & purification; pharmacology; Male; PPAR gamma; biosynthesis; genetics; Panax; chemistry; Plants, Medicinal; chemistry; RNA, Messenger; biosynthesis; genetics; Random Allocation; Rats; Rats, Wistar
- From: China Journal of Chinese Materia Medica 2006;31(15):1272-1276
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the mechanism of Shenqi compound recipe (SQCR) anti-earlier diabetic artherosclerosis in GK rats.
METHODFour-month specefic pathogen free (SPF) GK rats were divided randomly according to blood glucose level into four groups: model group (5 mL x kg(-1) x d(-1) sterile water), ramipril group (positive control, 1 mg x kg(-1) x d(-1)), SQCR low dosage (0.72 g x kg(-1) x d(-1)) and SQCR high dosage group (2.88 g x kg(-1) x d(-1)) and Wistar rats as normal control group(5 mL x kg(-1) x d(-1) sterile water). GK rats took high-fat diet freely and meanwile were injected N-omega-nitro-L-arginine methyl ester (L-N-AME) intra-peritoneally with the dose of 10 mg x kg(-1) x d(-1) in order to induce earlier diabetic artherosclerosis, while normal control group took regular diet and were injected normal saline intra-peritoneally. In the experiment periods, each group was administrated correspondent substance respectively for 32 d. At the end, sampling blood by abdominal aorta and picking aorta on ice. Determined monocyte chemoattractant protein-1 (MCP-1) concentration by ELISA, messenger ribonucleic acid (mRNA) expression of MCP-1 and peroxisome proliferator-activated receptor gamma (PPARgamma) in aorta by reverse transcriptase PCR (RT-PCR).
RESULTConcentrations of MCP-1 in serum in SQCR low and high dosage groups and the mRNA expression of MCP-1 in SQCR high dosage group were all decreased significantly compared with model group (P < 0.05). The mRNA expression of PPARgamma in SQCR low and high dosage groups all increased compared with model group (P < 0.05 or P < 0.01).
CONCLUSIONInhibiting the mRNA and protein expression of MCP-1 and upregulating the mRNA expression of PPARgamma in aorta might be contribute to SQCR anti-earlier diabetic artherosclerosis in GK rats partly.