Determination of cyclovirobuxine-D in huangyangning tablets by RP-HPLC with gradient elution.
- Author:
Yong CHEN
1
;
Xue-Jun FAN
;
Qing GONG
Author Information
- Publication Type:Journal Article
- MeSH: Buxus; chemistry; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; administration & dosage; analysis; chemistry; isolation & purification; Plants, Medicinal; chemistry; Tablets
- From: China Journal of Chinese Materia Medica 2006;31(14):1155-1157
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a new method for the determination of cyclovirobuxine-D in huangyangning tablets.
METHODAgilent Zorbax Extend C18 (4.6 mm x 250 mm, 5 microm) column with temperature 40 degrees C was used. The mobile phase was 0.3% diethylamine methanol solution and 0.3% diethylamine water solution, with a linear gradient of 0.3% diethylamine methanol solution from 78% to 95% in 9 minutes, and then maintained for 6 minutes. The flow rate was 0.8 mL x min(-1). Evaporative light scattering detector was used.
RESULTThe calibration curve was linear at a range of 0.57-11.44 microg for the cyclovirobuxine D (r = 0.9996). The average recovery was 97.2% and RSD was 1.1% (n = 9).
CONCLUSIONThis method is rapid, simple, and reproducible, and can be used as a quality control method for this preparation.
