Determination of three kind of diester diterpenoid alkaloids (DDAs) in Aconitum carmichaeli and its processed products by HPLC.
- Author:
Fang LIU
1
;
Xiang-Hong YU
;
Fei LI
;
Yu-Yuan TAN
;
Yan-Jiang QIAO
Author Information
- Publication Type:Journal Article
- MeSH: Aconitine; analogs & derivatives; analysis; Aconitum; chemistry; Alkaloids; analysis; Chromatography, High Pressure Liquid; methods; Hot Temperature; Plant Roots; chemistry; Plants, Medicinal; chemistry; Quality Control; Reproducibility of Results; Technology, Pharmaceutical; methods
- From: China Journal of Chinese Materia Medica 2006;31(14):1160-1162
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish an HPLC method for the determination of three kind of diester diterpenoid alkaloids (DDAs) in Aconitum carmichaeli and its processed products namely mesaconitine, aconitine and hypaconitine.
METHODA Zorbax Eclipse XDB C18 column was used, and ammonium acetate solution-acetonitrile as the mobile phase, with a flow rate of 1.0 mL x min(-1) and a detection wavelength of 230 nm.
RESULTMesaconitine, aconitine and hypaconitine, were separated, and the calibration curves of them were in good linearity over the range of 0.035 7-1.784 microg (r = 0.9999), 0.0126-0.632 microg (r = 0.9997) and 0.0334-1.672 microg (r = 0.9997) respectively.
CONCLUSIONThis method is simple and accurate. It can be used in the identification and quality control of A. carmichaeli.
