Study on separation and purification of anthraquinones in radix et rhizoma rhei by D301 macroporous resin.
- Author:
Han-Lin XU
1
;
Jun CHEN
;
Ji-Zhen SHAO
;
Yun SUN
;
Chi-Zhi ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Anthraquinones; isolation & purification; Chromatography, High Pressure Liquid; Plant Roots; chemistry; Plants, Medicinal; chemistry; Resins, Synthetic; Rheum; chemistry; Rhizome; chemistry
- From: China Journal of Chinese Materia Medica 2006;31(14):1163-1165
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThe parameters of absorption and purification of total anthraquinones in Radix Et Rhizoma Rhei by D301 macroporous resin were investigated in this paper.
METHODHPLC was used to analyze the content of total anthraquinones in Radix Et Rhizoma with emodin as control.
RESULTThe appropriate adsorption conditions were: concentration of extract 0.5 g x mL(-1); pH 9; flow rate 1 BV x h(-1). When the 75% ethanol was used as elution and the concentration of HCl was 0.1 mol x L(-1), and the flow rate was 1.0 BV x h(-1), the effect of desorption was satisfactory.
CONCLUSIOND301 resin provided a good effect on the exchang and absorption of total anthraquinones in Radix Et Rhizoma.
