Effect of Rongshi granule on renal stone formation and osteopontin expression in rat urolithiasis model.
- Author:
Ping HUANG
1
;
Su-Wen YANG
;
Wei-Hua HUANG
;
Fan-Zhi KONG
;
Yi-Jia LOU
Author Information
- Publication Type:Journal Article
- MeSH: Ammonium Chloride; Animals; Calcium; urine; Calcium Oxalate; metabolism; Dose-Response Relationship, Drug; Drug Combinations; Drugs, Chinese Herbal; administration & dosage; isolation & purification; pharmacology; Ethylene Glycol; Female; Kidney; metabolism; Kidney Calculi; chemically induced; metabolism; Male; Osteopontin; metabolism; Oxalic Acid; urine; Plants, Medicinal; chemistry; Random Allocation; Rats; Rats, Sprague-Dawley
- From: China Journal of Chinese Materia Medica 2006;31(14):1188-1191
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of Rongshi granule on osteopontin(OPN) expression.
METHODThe urlisthiasis rats were induced by ethylene glycol (EG) and ammonium chloride, the control group rats were non-treated, and the Rongshi granule groups (low-dose group, middle-dose group and high-dose group) were administered Rongshi granule in addition to EG and ammonium chloride in 21 days. Pooled 24 h urine samples from each group were collected weekly with the use of metabolic cages, the concentration of uric calcium and oxalic acid were respectively measured by EDTA and photoelectric colorimetric method. Eight animals from each group were killed at 0, 7, 14, and 21 days, kidneys were histologic examinaed and immunohistochemical staining.
RESULTThe expression of kidney osteopontin in model group was obviously higher than that of control group (P <0.01), and was up to the highest at 21 days with 1.4 times (0.281 3/0.201 8) of the control group. The expression of kidney osteopontin in all of the Rongshi granule groups were lower than those of model group (P < 0.05), with an obvious dose-dependent manner. The degree of the kidney calcium oxalate crystal of the rats in all the Rongshi granule groups was much lower than that of model group, and the uric calcium and oxalic acid were much lower than those of model group (P < 0.01).
CONCLUSIONThe Rongshi granule could inhibit the expression of osteopontin in rat urolithiasis model.
