Studies on HPLC fingerprint of Radix Aconiti Kusnezoffii.
- Author:
Ying-yong ZHAO
1
;
Xiu-ming CUI
;
Yun DAI
;
Hua MIAO
Author Information
- Publication Type:Journal Article
- MeSH: Aconitine; analogs & derivatives; analysis; Aconitum; chemistry; Chromatography, High Pressure Liquid; methods; Plant Tubers; chemistry; Plants, Medicinal; chemistry; Quality Control; Reproducibility of Results
- From: China Journal of Chinese Materia Medica 2006;31(13):1056-1058
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish the HPLC fingerprint of Radix Aconiti Kusnezoffii.
METHODThe chromatographic separation was performed on a Kromasil C18 (4.6 mm x 250 mm, 5 microm) eluted with a mobile phase of acetonitrile-ammonium acetate buffer (2. 5 per thousand acetic acid-ammonia pH 10.5) (60:40). The UV detection wavelength was set at 240 nm and the flow rate was set at 1.0 mL x min(-1).
RESULTThe RSD of precision and repeatability was less than 2%. Under the selected chromatographic conditions, good HPLC fingerprints of Radix Aconiti Kusnezoffii were obtained.
CONCLUSIONThe method was simple, accurate and repeatable. It can be used for the quality control of Radix Aconiti Kusnezoffii.
